4E0D
Binary complex of Bacillus DNA Polymerase I Large Fragment E658A and duplex DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Detector technology | CCD |
Collection date | 2011-01-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1159 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 87.860, 93.780, 104.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.703 - 1.580 |
R-factor | 0.1621 |
Rwork | 0.161 |
R-free | 0.18120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4DQQ chains A B and C |
RMSD bond length | 0.010 |
RMSD bond angle | 1.335 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1026) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 1.670 |
High resolution limit [Å] | 1.580 | 4.730 | 1.580 |
Rmerge | 0.059 | 0.026 | 0.476 |
Number of reflections | 117529 | ||
<I/σ(I)> | 24.7 | 68.54 | 4.33 |
Completeness [%] | 98.8 | 99.7 | 98.9 |
Redundancy | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | 47% Saturated Ammonium Sulfate, 2.5% MPD, 10mM MgSO4, 100mM MES (pH 5.8), VAPOR DIFFUSION, HANGING DROP, temperature 290K |