4CDN

Crystal structure of M. mazei photolyase with its in vivo reconstituted 8-HDF antenna chromophore

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DEOXYRIBODIPYRIMIDINE PHOTOLYASE	polymer	482	55123.5	2	UniProt (Q8PYK9) Pfam (PF00875) In PDB	METHANOSARCINA MAZEI	CLASS II CPD PHOTOLYASE
2	A, B	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)
3	A, B	1-deoxy-1-(8-hydroxy-2,4-dioxo-3,4-dihydropyrimido[4,5-b]quinolin-10(2H)-yl)-D-ribitol	non-polymer		363.3	2	Chemie (FO1)
4	A, B	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
5	A, B	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
6	A	TRIETHYLENE GLYCOL	non-polymer		150.2	2	Chemie (PGE)
7	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	4	Chemie (PEG)
8		water	water		18.0	690	Chemie (HOH)

Sequence modifications

A, B: 3 - 464 (UniProt: Q8PYK9)

PDB	External Database	Details
Met -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Ser -7	-	expression tag
Ser -6	-	expression tag
Gly -5	-	expression tag
Leu -4	-	expression tag
Val -3	-	expression tag
Pro -2	-	expression tag
Arg -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
His 2	-	expression tag
Thr 377	Met 377	cloning artifact

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	110247.0
Non-Polymers*	Number of molecules	18
	Total formula weight	3771.2
All*	Total formula weight	114018.2

*Water molecules are not included.