4CDN
Crystal structure of M. mazei photolyase with its in vivo reconstituted 8-HDF antenna chromophore
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-30 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.970, 114.740, 141.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.470 - 1.900 |
| R-factor | 0.14916 |
| Rwork | 0.149 |
| R-free | 0.17844 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xry |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.354 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.040 | 0.430 |
| Number of reflections | 99823 | |
| <I/σ(I)> | 23.3 | 3.2 |
| Completeness [%] | 98.5 | 98.7 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M LITHIUM SULFATE, 0.1 M TRI-SODIUM CITRATE PH 5.6, 12% (W/V) PEG 6000 |
