4C2A

Crystal Structure of High-Affinity von Willebrand Factor A1 domain with R1306Q and I1309V Mutations in Complex with High Affinity GPIb alpha

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	VON WILLEBRAND FACTOR	polymer	215	24669.6	1	UniProt (P04275) Pfam (PF00092)	HOMO SAPIENS (HUMAN)	VWF, VON WILLEBRAND ANTIGEN II, VON WILLEBRAND FACTOR A1
2	B (B)	PLATELET GLYCOPROTEIN IB ALPHA CHAIN	polymer	291	32492.1	1	UniProt (P07359) Pfam (PF01462) Pfam (PF13855)	HOMO SAPIENS (HUMAN)	GP-IB ALPHA, GPIB-ALPHA, GPIBA, GLYCOPROTEIN IB ALPHA, ANTIGEN CD42B-ALPHA
3	C (A)	CACODYLATE ION	non-polymer		137.0	1	Chemie (CAC)
4	D (A)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
5	E (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
6	F (B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
7	G, H (A, B)	water	water		18.0	204	Chemie (HOH)

Sequence modifications

A: 1264 - 1471 (UniProt: P04275)

PDB	External Database	Details
Met 1263	-	expression tag
His 1472	-	expression tag
His 1473	-	expression tag
His 1474	-	expression tag
His 1475	-	expression tag
His 1476	-	expression tag
His 1477	-	expression tag
Gln 1306	Arg 1306	engineered mutation
Val 1309	Ile 1309	engineered mutation
Ala 1381	Thr 1381	variant

B: 1 - 290 (UniProt: P07359)

PDB	External Database	Details
Arg 21	Asn 37	engineered mutation
Arg 159	Asn 175	engineered mutation
Val 233	Gly 249	engineered mutation
Val 239	Met 255	engineered mutation
Gly 291	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	57161.7
Non-Polymers*	Number of molecules	4
	Total formula weight	342.2
All*	Total formula weight	57503.9

*Water molecules are not included.