4C2A
Crystal Structure of High-Affinity von Willebrand Factor A1 domain with R1306Q and I1309V Mutations in Complex with High Affinity GPIb alpha
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | VON WILLEBRAND FACTOR | polymer | 215 | 24669.6 | 1 | UniProt (P04275) Pfam (PF00092) In PDB | HOMO SAPIENS (HUMAN) | VWF, VON WILLEBRAND ANTIGEN II, VON WILLEBRAND FACTOR A1 |
2 | B | PLATELET GLYCOPROTEIN IB ALPHA CHAIN | polymer | 291 | 32492.1 | 1 | UniProt (P07359) Pfam (PF01462) Pfam (PF13855) In PDB | HOMO SAPIENS (HUMAN) | GP-IB ALPHA, GPIB-ALPHA, GPIBA, GLYCOPROTEIN IB ALPHA, ANTIGEN CD42B-ALPHA |
3 | A | CACODYLATE ION | non-polymer | 137.0 | 1 | Chemie (CAC) | |||
4 | A | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
5 | A | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
6 | B | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
7 | water | water | 18.0 | 204 | Chemie (HOH) |
Sequence modifications
A: 1264 - 1471 (UniProt: P04275)
B: 1 - 290 (UniProt: P07359)
PDB | External Database | Details |
---|---|---|
Met 1263 | - | expression tag |
His 1472 | - | expression tag |
His 1473 | - | expression tag |
His 1474 | - | expression tag |
His 1475 | - | expression tag |
His 1476 | - | expression tag |
His 1477 | - | expression tag |
Gln 1306 | Arg 1306 | engineered mutation |
Val 1309 | Ile 1309 | engineered mutation |
Ala 1381 | Thr 1381 | variant |
PDB | External Database | Details |
---|---|---|
Arg 21 | Asn 37 | engineered mutation |
Arg 159 | Asn 175 | engineered mutation |
Val 233 | Gly 249 | engineered mutation |
Val 239 | Met 255 | engineered mutation |
Gly 291 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 57161.7 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 342.2 | |
All* | Total formula weight | 57503.9 |