4BXF

60S ribosomal protein L27A histidine hydroxylase (MINA53 Y209C) in complex with MN(II), 2-oxoglutarate (2OG) and 60S ribosomal protein L27A (RPL27A G37C) peptide fragment

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	BIFUNCTIONAL LYSINE-SPECIFIC DEMETHYLASE AND HISTIDYL-HYDROXYLASE MINA	polymer	442	50372.2	2	UniProt (Q8IUF8) Pfam (PF08007) Pfam (PF20514)	HOMO SAPIENS (HUMAN)	60S RIBOSOMAL PROTEIN L27A HISTIDINE HYDROXYLASE, HISTONE LYSINE DEMETHYLASE MINA, MYC-INDUCED NUCLEAR ANTIGEN, MINERAL DUST-INDUCED GENE PROTEIN, NUCLEOLAR PROTEIN 52, RIBOSOMAL OXYGENASE MINA, ROX, MYC-INDUCED NUCLEAR ANTIGEN
2	C, D (C, D)	60S RIBOSOMAL PROTEIN L27A	polymer	19	2279.6	2	UniProt (P46776)	HOMO SAPIENS
3	E, G (A, B)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
4	F, H (A, B)	2-OXOGLUTARIC ACID	non-polymer		146.1	2	Chemie (AKG)
5	I, J, K, L (A, B, C, D)	water	water		18.0	332	Chemie (HOH)

Sequence modifications

A, B: 26 - 465 (UniProt: Q8IUF8)

PDB	External Database	Details
Ser 24	-	expression tag
Met 25	-	expression tag
Cys 209	Tyr 209	engineered mutation

C, D: 32 - 50 (UniProt: P46776)

PDB	External Database	Details
Cys 37	Gly 37	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	105303.6
Non-Polymers*	Number of molecules	4
	Total formula weight	402.1
All*	Total formula weight	105705.6

*Water molecules are not included.