4BPX

Crystal structure of human primase in complex with the primase- binding motif of DNA polymerase alpha

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	DNA PRIMASE SMALL SUBUNIT	polymer	423	50108.0	2	UniProt (P49642) Pfam (PF01896)	HOMO SAPIENS (HUMAN)	DNA PRIMASE 49 KDA SUBUNIT, P49
2	B, D (B, D)	DNA POLYMERASE ALPHA CATALYTIC SUBUNIT, DNA PRIMASE LARGE SUBUNIT	polymer	269	30774.1	2	UniProt (P09884) UniProt (P49643)	HOMO SAPIENS (HUMAN)	DNA POLYMERASE ALPHA CATALYTIC SUBUNIT P180, DNA PRIMASE 58 KDA SUBUNIT, P58
3	E, F (A, C)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A, C: 1 - 420 (UniProt: P49642)

PDB	External Database	Details
Gly -2	-	expression tag
Thr -1	-	expression tag
Ser 0	-	expression tag
Ala 72	Lys 72	engineered mutation
Ala 73	Met 73	engineered mutation

B, D: 1445 - 1462 (UniProt: P09884)

PDB	External Database	Details
Met 1444	-	expression tag

B, D: 19 - 253 (UniProt: P49643)

PDB	External Database	Details
Thr 4	-	linker
Gly 5	-	linker
Ser 6	-	linker
Thr 7	-	linker
Gly 8	-	linker
Ser 9	-	linker
Thr 10	-	linker
Gly 11	-	linker
Ser 12	-	linker
Thr 13	-	linker
Gly 14	-	linker
Ser 15	-	linker
Thr 16	-	linker
Gly 17	-	linker
Ser 18	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	161764.1
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	161895.0

*Water molecules are not included.