4BPX
Crystal structure of human primase in complex with the primase- binding motif of DNA polymerase alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 120.680, 70.701, 127.160 |
| Unit cell angles | 90.00, 105.83, 90.00 |
Refinement procedure
| Resolution | 45.325 - 3.400 |
| R-factor | 0.2361 |
| Rwork | 0.234 |
| R-free | 0.26690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bpu |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.703 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.370 | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.060 | 1.500 |
| Number of reflections | 28805 | |
| <I/σ(I)> | 13.8 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.2 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100MM TRIS-HCL/BICINE PH 8.5, 20% GLYCEROL, 10% PEG4000 AND 20MM EACH OF AN ALCOHOL MIX COMPRISING 1,6-HEXANEDIOL, 1-BUTANOL, 1,2-PROPANEDIOL, 2-PROPANOL, 1,4- BUTANEDIOL AND 1,3-PROPANEDIOL. |
