4BLW
Crystal structure of Escherichia coli 23S rRNA (A2030-N6)- methyltransferase RlmJ in complex with S-adenosylhomocysteine (AdoHcy) and Adenosine monophosphate (AMP)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | RIBOSOMAL RNA LARGE SUBUNIT METHYLTRANSFERASE J | polymer | 289 | 33088.9 | 2 | UniProt (P37634) Pfam (PF04378) | ESCHERICHIA COLI | |
| 2 | C, R (A, B) | ADENOSINE MONOPHOSPHATE | non-polymer | 347.2 | 2 | Chemie (AMP) PubChem (16397049) PubChem (16760266) PubChem (224) PubChem (34768) PubChem (83862) PubChem (40467049) PubChem (40469468) PubChem (446048) PubChem (44348067) PubChem (45358029) PubChem (40459109) PubChem (515384) PubChem (46874558) PubChem (638042) PubChem (46875232) PubChem (57358045) PubChem (57879893) PubChem (57879902) PubChem (5316444) PubChem (5742864) PubChem (177678969) PubChem (6083) PubChem (1549371) PubChem (12163610) PubChem (12358355) PubChem (92144436) PubChem (122197642) PubChem (12358354) PubChem (162290139) PubChem (162946266) PubChem (171699505) | |||
| 3 | D, S (A, B) | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 2 | Chemie (SAH) PubChem (16760024) PubChem (193) PubChem (13792) PubChem (177678424) PubChem (189149) PubChem (439155) PubChem (25210495) PubChem (21125603) PubChem (44361184) PubChem (25203782) PubChem (46936206) PubChem (50989525) PubChem (51380914) PubChem (52224398) PubChem (57544532) PubChem (52224400) PubChem (59046843) PubChem (59170628) PubChem (59576228) PubChem (66805708) PubChem (5284331) PubChem (70689219) PubChem (70702054) PubChem (70832431) PubChem (71170554) PubChem (13231890) PubChem (6419189) PubChem (118374891) PubChem (118560068) PubChem (126969526) PubChem (129105892) PubChem (164157219) PubChem (143518505) PubChem (154578335) PubChem (163054214) PubChem (163335525) | |||
| 4 | E, F, T (A, B) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) PubChem (753) | |||
| 5 | G, H, I, J, K... (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 14 | Chemie (EDO) | |||
| 6 | P, Y (A, B) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) PubChem (923) | |||
| 7 | Q (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 8 | AA, BA (A, B) | water | water | 18.0 | 335 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 280 (UniProt: P37634)
| PDB | External Database | Details |
|---|---|---|
| Ser 281 | - | expression tag |
| Lys 282 | - | expression tag |
| Gly 283 | - | expression tag |
| His 284 | - | expression tag |
| His 285 | - | expression tag |
| His 286 | - | expression tag |
| His 287 | - | expression tag |
| His 288 | - | expression tag |
| His 289 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 66177.7 | |
| Non-Polymers* | Number of molecules | 24 |
| Total formula weight | 2750.5 | |
| All* | Total formula weight | 68928.3 |
