4BLW

Crystal structure of Escherichia coli 23S rRNA (A2030-N6)- methyltransferase RlmJ in complex with S-adenosylhomocysteine (AdoHcy) and Adenosine monophosphate (AMP)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	RIBOSOMAL RNA LARGE SUBUNIT METHYLTRANSFERASE J	polymer	289	33088.9	2	UniProt (P37634) Pfam (PF04378) In PDB	ESCHERICHIA COLI
2	A, B	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	2	Chemie (AMP)
3	A, B	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH)
4	A, B	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
5	A, B	1,2-ETHANEDIOL	non-polymer		62.1	14	Chemie (EDO)
6	A, B	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
7	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
8		water	water		18.0	335	Chemie (HOH)

Sequence modifications

A, B: 1 - 280 (UniProt: P37634)

PDB	External Database	Details
Ser 281	-	expression tag
Lys 282	-	expression tag
Gly 283	-	expression tag
His 284	-	expression tag
His 285	-	expression tag
His 286	-	expression tag
His 287	-	expression tag
His 288	-	expression tag
His 289	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	66177.7
Non-Polymers*	Number of molecules	24
	Total formula weight	2750.5
All*	Total formula weight	68928.3

*Water molecules are not included.