4BBN

NEDD4 HECT-Ub:Ub complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	E3 UBIQUITIN-PROTEIN LIGASE NEDD4	polymer	385	45630.9	1	UniProt (P46934) Pfam (PF00632) In PDB	HOMO SAPIENS (HUMAN)	CELL PROLIFERATION-INDUCING GENE 53 PROTEIN, NEURAL PRECURSOR CELL EXPRESSED DEVELOPMENTALLY DOWN-REGULATED PROTEIN 4, NEDD-4
2	C	POLYUBIQUITIN-B	polymer	76	8576.8	1	UniProt (P0CG53) Pfam (PF00240) In PDB	BOS TAURUS (CATTLE)	UBIQUITIN
3	F	POLYUBIQUITIN-B	polymer	76	8622.9	1	UniProt (P0CG53) Pfam (PF00240) In PDB	BOS TAURUS (CATTLE)	UBIQUITIN
4		water	water		18.0	110	Chemie (HOH)

Sequence modifications

A: 515 - 899 (UniProt: P46934)

PDB	External Database	Details
Gly 515	-	expression tag
Pro 516	-	expression tag
Leu 517	-	expression tag
Gly 518	-	expression tag
Ile 626	Leu 1045	engineered mutation
-	Cys 1046	deletion
Pro 628	Glu 1048	engineered mutation
Ser 777	Cys 1197	engineered mutation

F: 1 - 76 (UniProt: P0CG53)

PDB	External Database	Details
Cys 76	Gly 304	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	62830.7
All*	Total formula weight	62830.7

*Water molecules are not included.