4BBN
NEDD4 HECT-Ub:Ub complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-03 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 196.540, 196.540, 98.770 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 98.270 - 2.510 |
| R-factor | 0.17852 |
| Rwork | 0.176 |
| R-free | 0.22443 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xbb |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.142 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.270 | 2.570 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Rmerge | 0.090 | 0.880 |
| Number of reflections | 25322 | |
| <I/σ(I)> | 16.6 | 2.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 2.5 SODIUM MALONATE, PH 6.0 |
