4AP6

Crystal structure of human POFUT2 E54A mutant in complex with GDP- fucose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	GDP-FUCOSE PROTEIN O-FUCOSYLTRANSFERASE 2	polymer	422	49554.0	4	UniProt (Q9Y2G5) Pfam (PF10250)	HOMO SAPIENS (HUMAN)	PEPTIDE-O-FUCOSYLTRANSFERASE 2, O-FUCT-2, PROTEIN O-FUCOSYLTRANSFERASE 2
2	E, H, M, Q (A, B, C, D)	GUANOSINE-5'-DIPHOSPHATE-BETA-L-FUCOPYRANOSE	non-polymer		589.3	4	Chemie (GFB)
3	F, I, J, K, N... (A, B, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)
4	G, L, P, R (A, B, C, D)	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
5	S, T (A, B)	water	water		18.0	8	Chemie (HOH)

Sequence modifications

A, B, C, D: 37 - 429 (UniProt: Q9Y2G5)

PDB	External Database	Details
Ser 8	-	expression tag
Met 9	-	expression tag
Ala 10	-	expression tag
Ser 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
Ser 18	-	expression tag
Ser 19	-	expression tag
Gly 20	-	expression tag
Asp 21	-	expression tag
Tyr 22	-	expression tag
Lys 23	-	expression tag
Asp 24	-	expression tag
Asp 25	-	expression tag
Asp 26	-	expression tag
Asp 27	-	expression tag
Lys 28	-	expression tag
Leu 29	-	expression tag
Glu 30	-	expression tag
Val 31	-	expression tag
Leu 32	-	expression tag
Phe 33	-	expression tag
Gln 34	-	expression tag
Gly 35	-	expression tag
Pro 36	-	expression tag
Ala 54	Glu 54	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	198215.8
Non-Polymers*	Number of molecules	14
	Total formula weight	3826.4
All*	Total formula weight	202042.3

*Water molecules are not included.