4AP6
Crystal structure of human POFUT2 E54A mutant in complex with GDP- fucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 153.010, 153.010, 185.680 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.686 - 3.401 |
R-factor | 0.1947 |
Rwork | 0.192 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.292 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.630 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.170 | 0.770 |
Number of reflections | 32751 | |
<I/σ(I)> | 11 | 2.1 |
Completeness [%] | 93.3 | 93.6 |
Redundancy | 4.7 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 20% PEG 3350, 0.2M NASCN |