4ADQ

CRYSTAL STRUCTURE OF THE MOUSE COLONY-STIMULATING FACTOR 1 (MCSF-1) CYTOKINE IN COMPLEX WITH THE VIRAL RECEPTOR BARF1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	SECRETED PROTEIN BARF1	polymer	208	23412.7	4	UniProt (P0CW72) Pfam (PF22305) UniProt (by SIFTS) (P03228)	Human gammaherpesvirus 4	BARF1,33 KDA EARLY PROTEIN, P33
2	E, F, G, H (E, F, G, H)	MACROPHAGE COLONY-STIMULATING FACTOR 1	polymer	153	17872.1	4	UniProt (P07141) Pfam (PF05337)	Mus musculus (house mouse)	CSF-1, M-CSF, MCSF, PROCESSED MACROPHAGE COLONY-STIMULATING FACTOR 1
3	I, K, L (I, K, L)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	3	In PDB GlyTouCan (G15407YE)
4	J (J)	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G81315DD)

Sequence modifications

A, B, C, D: 21 - 221 (UniProt: P0CW72)

PDB	External Database	Details
Lys 222	-	expression tag
His 223	-	expression tag
His 224	-	expression tag
His 225	-	expression tag
His 226	-	expression tag
His 227	-	expression tag
His 228	-	expression tag
Ser 169	Thr 169	engineered mutation

E, F, G, H: 1 - 149 (UniProt: P07141)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	165139.0
Branched	Number of molecules	4
	Total formula weight	2508.3
All*	Total formula weight	167647.4

*Water molecules are not included.