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4ADQ

CRYSTAL STRUCTURE OF THE MOUSE COLONY-STIMULATING FACTOR 1 (MCSF-1) CYTOKINE IN COMPLEX WITH THE VIRAL RECEPTOR BARF1

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID29
Synchrotron siteESRF
BeamlineID29
Temperature [K]100
Detector technologyPIXEL
Collection date2011-05-08
DetectorDECTRIS PILATUS 6M
Spacegroup nameH 3
Unit cell lengths234.750, 234.750, 96.040
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution117.375 - 4.500
R-factor0.2265
Rwork0.223
R-free0.26220
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)PDB ENTRIES 2CH8 AND 3UF5
RMSD bond length0.003
RMSD bond angle0.713
Data reduction softwareXDS
Data scaling softwareSCALA
Phasing softwarePHASER
Refinement softwarePHENIX ((PHENIX.REFINE))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]40.0004.620
High resolution limit [Å]4.5004.500
Rmerge0.1500.680
Number of reflections11082
<I/σ(I)>5.71.6
Completeness [%]94.593.2
Redundancy1.71.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
18pH 8.0

229183

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