4A0K

STRUCTURE OF DDB1-DDB2-CUL4A-RBX1 BOUND TO A 12 BP ABASIC SITE CONTAINING DNA-DUPLEX

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	CULLIN-4A	polymer	742	86702.8	1	UniProt (Q13619) Pfam (PF00888) Pfam (PF10557)	HOMO SAPIENS	CULLIN HOMOLOG 4A, CUL-4A
2	B (B)	E3 UBIQUITIN-PROTEIN LIGASE RBX1	polymer	117	13626.4	1	UniProt (P62878) Pfam (PF12678)	MUS MUSCULUS (HOUSE MOUSE)	RING FINGER PROTEIN 75, RING-BOX PROTEIN 1, RBX1
3	C (C)	DNA DAMAGE-BINDING PROTEIN 1	polymer	1159	129394.9	1	UniProt (Q16531) Pfam (PF10433) Pfam (PF03178)	HOMO SAPIENS	DDB P127 SUBUNIT, DNA DAMAGE-BINDING PROTEIN A, DDBA, DAMAGE-SPECIFIC DNA-BINDING PROTEIN 1, HBV X-ASSOCIATED PROTEIN 1, XAP-1, UV-DAMAGED DNA-BINDING FACTOR, UV-DAMAGED DNA-BINDING PROTEIN 1, UV-DDB 1, XPE-BINDING FACTOR, XPE-BF, XERODERMA PIGMENTOSUM GROUP E-COMPLEMENTING PROTEIN, XPCE
4	D (D)	DNA DAMAGE-BINDING PROTEIN 2	polymer	382	43418.1	1	UniProt (Q2YDS1) Pfam (PF00400)	DANIO RERIO (ZEBRAFISH)	DAMAGE-SPECIFIC DNA-BINDING PROTEIN 2
5	E (E)	12 BP THF CONTAINING DNA	polymer	12	3498.3	1		SYNTHETIC CONSTRUCT
6	F (F)	12 BP DNA	polymer	12	3702.4	1		SYNTHETIC CONSTRUCT

Sequence modifications

A: 38 - 759 (UniProt: Q13619)

PDB	External Database	Details
Met 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
Val 25	-	expression tag
Asp 26	-	expression tag
Glu 27	-	expression tag
Glu 28	-	expression tag
Asn 29	-	expression tag
Leu 30	-	expression tag
Tyr 31	-	expression tag
Phe 32	-	expression tag
Gln 33	-	expression tag
Gly 34	-	expression tag
Gly 35	-	expression tag
Gly 36	-	expression tag
Arg 37	-	expression tag

B: 12 - 108 (UniProt: P62878)

PDB	External Database	Details
Met -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Val -1	-	expression tag
Asp 0	-	expression tag
Glu 1	-	expression tag
Glu 2	-	expression tag
Asn 3	-	expression tag
Leu 4	-	expression tag
Tyr 5	-	expression tag
Phe 6	-	expression tag
Gln 7	-	expression tag
Gly 8	-	expression tag
Gly 9	-	expression tag
Gly 10	-	expression tag
Arg 11	-	expression tag

C: 1 - 1140 (UniProt: Q16531)

PDB	External Database	Details
Met -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

D: 94 - 457 (UniProt: Q2YDS1)

PDB	External Database	Details
Met 76	-	expression tag
His 77	-	expression tag
His 78	-	expression tag
His 79	-	expression tag
His 80	-	expression tag
His 81	-	expression tag
His 82	-	expression tag
Arg 83	-	expression tag
Arg 84	-	expression tag
Leu 85	-	expression tag
Val 86	-	expression tag
Pro 87	-	expression tag
Arg 88	-	expression tag
Gly 89	-	expression tag
Ser 90	-	expression tag
Gly 91	-	expression tag
Gly 92	-	expression tag
Arg 93	-	expression tag
Gln 180	Leu 146	variant
Arg 214	Trp 180	variant

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	280342.9
All*	Total formula weight	280342.9

*Water molecules are not included.