4A0K
STRUCTURE OF DDB1-DDB2-CUL4A-RBX1 BOUND TO A 12 BP ABASIC SITE CONTAINING DNA-DUPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-07-30 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 210.650, 78.020, 276.620 |
| Unit cell angles | 90.00, 108.50, 90.00 |
Refinement procedure
| Resolution | 19.977 - 5.930 |
| R-factor | 0.2691 |
| Rwork | 0.269 |
| R-free | 0.26950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2HYE AND 3EI2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.403 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER (MOLREP) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 6.080 |
| High resolution limit [Å] | 5.930 | 5.930 |
| Rmerge | 0.140 | 0.550 |
| Number of reflections | 11289 | |
| <I/σ(I)> | 8.1 | 2.41 |
| Completeness [%] | 98.5 | 99.9 |
| Redundancy | 4.9 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.3 | 100MM TRIS-HCL PH 8.3, 33% PEG 200 |
