4A0B
Structure of hsDDB1-drDDB2 bound to a 16 bp CPD-duplex (pyrimidine at D-1 position) at 3.8 A resolution (CPD 4)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | DNA DAMAGE-BINDING PROTEIN 1 | polymer | 1159 | 129352.9 | 2 | UniProt (Q16531) Pfam (PF10433) Pfam (PF03178) | HOMO SAPIENS (HUMAN) | |
| 2 | B, D (B, D) | DNA DAMAGE-BINDING PROTEIN 2 | polymer | 382 | 43418.1 | 2 | UniProt (Q2YDS1) Pfam (PF00400) | DANIO RERIO (ZEBRAFISH) | DAMAGE-SPECIFIC DNA-BINDING PROTEIN 2 |
| 3 | E, G (G, I) | 5'-D(*DGP*GP*TP*GP*AP*AP*AP*(TTD)P*AP*GP*CP*AP*GP*DGP)-3' | polymer | 15 | 5018.3 | 2 | synthetic construct | ||
| 4 | F, H (H, J) | 5'-D(*CP*CP*TP*GP*CP*TP*CP*CP*TP*TP*TP*CP*AP*CP*CP*C)-3' | polymer | 16 | 4730.1 | 2 | synthetic construct |
Sequence modifications
A, C: 1 - 1140 (UniProt: Q16531)
B, D: 94 - 457 (UniProt: Q2YDS1)
| PDB | External Database | Details |
|---|---|---|
| Met -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| Val -11 | - | expression tag |
| Asp -10 | - | expression tag |
| Glu -9 | - | expression tag |
| Asn -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Tyr -6 | - | expression tag |
| Phe -5 | - | expression tag |
| Gln -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Arg 0 | - | expression tag |
| Ser 224 | Glu 224 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met 76 | - | expression tag |
| His 77 | - | expression tag |
| His 78 | - | expression tag |
| His 79 | - | expression tag |
| His 80 | - | expression tag |
| His 81 | - | expression tag |
| His 82 | - | expression tag |
| Arg 83 | - | expression tag |
| Arg 84 | - | expression tag |
| Leu 85 | - | expression tag |
| Val 86 | - | expression tag |
| Pro 87 | - | expression tag |
| Arg 88 | - | expression tag |
| Gly 89 | - | expression tag |
| Ser 90 | - | expression tag |
| Gly 91 | - | expression tag |
| Gly 92 | - | expression tag |
| Arg 93 | - | expression tag |
| Gln 180 | Leu 146 | variant |
| Arg 214 | Trp 180 | variant |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 365038.6 | |
| All* | Total formula weight | 365038.6 |
