4A0B
Structure of hsDDB1-drDDB2 bound to a 16 bp CPD-duplex (pyrimidine at D-1 position) at 3.8 A resolution (CPD 4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-11-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 113.100, 145.900, 224.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.720 - 3.800 |
| R-factor | 0.24719 |
| Rwork | 0.243 |
| R-free | 0.31889 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ei1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.123 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.900 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.140 | 0.620 |
| Number of reflections | 37310 | |
| <I/σ(I)> | 11.9 | 3.2 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 7.4 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.3 | 100 MM MES, 15 MM NAOH, 18% PEG 350MME., pH 5.3 |
