43NO

Crystal structure of a de novo-designed Binder targeting VEGFA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, E, F (A, B, E, F)	VEGFA_Binder-004	polymer	130	14860.1	4		synthetic construct
2	C, D, G, H (C, D, G, H)	Vascular endothelial growth factor A, long form	polymer	104	12331.2	4	UniProt (P15692) Pfam (PF00341)	Homo sapiens (human)	L-VEGF,Vascular permeability factor,VPF
3	I, J (C, G)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL) PubChem (753)
4	K, L, M, N, O... (A, B, C, D, E...)	water	water		18.0	91	Chemie (HOH)

Sequence modifications

C, D, G, H: 219 - 313 (UniProt: P15692)

PDB	External Database	Details
Met 218	-	initiating methionine
Leu 314	-	expression tag
Glu 315	-	expression tag
His 316	-	expression tag
His 317	-	expression tag
His 318	-	expression tag
His 319	-	expression tag
His 320	-	expression tag
His 321	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	108765.2
Non-Polymers*	Number of molecules	2
	Total formula weight	184.2
All*	Total formula weight	108949.4

*Water molecules are not included.