3WZM

ZEN lactonase mutant complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Zearalenone hydrolase	polymer	278	30482.4	3	UniProt (Q8NKB0) Pfam (PF00561) In PDB	Clonostachys rosea
2	A, B, C	(3S,11E)-14,16-dihydroxy-3-methyl-3,4,5,6,9,10-hexahydro-1H-2-benzoxacyclotetradecine-1,7(8H)-dione	non-polymer		318.4	3	Chemie (ZER)
3		water	water		18.0	426	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 264 (UniProt: Q8NKB0)

PDB	External Database	Details
Met -13	-	expression tag
Ala -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Val -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag
Ala 102	Ser 102	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	91447.3
Non-Polymers*	Number of molecules	3
	Total formula weight	955.1
All*	Total formula weight	92402.4

*Water molecules are not included.