3WZM
ZEN lactonase mutant complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 86.842, 86.842, 471.784 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.950 - 2.480 |
| R-factor | 0.20736 |
| Rwork | 0.204 |
| R-free | 0.26788 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 3WZL |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.763 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.570 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.073 | 0.345 |
| Number of reflections | 38626 | |
| <I/σ(I)> | 34 | 6.2 |
| Completeness [%] | 99.4 | 97.8 |
| Redundancy | 6.2 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 24% PEG 2000 MME, 0.1M Bis-Tris pH 6.5, Soaking with Reservoir + 10mM zearalenone) , VAPOR DIFFUSION, SITTING DROP, temperature 298K |
