3WXM

Crystal structure of archaeal Pelota and GTP-bound EF1 alpha complex

Replaces:  3AGJ

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G (A, C, E, G)	Elongation factor 1-alpha	polymer	447	49662.6	4	UniProt (Q9YAV0) Pfam (PF00009) Pfam (PF03144) Pfam (PF22594)	Aeropyrum pernix K1	GTP-bound aEF1 alpha, EF-1-alpha, Elongation factor Tu, EF-Tu
2	B, D, F, H (B, D, F, H)	Protein pelota homolog	polymer	376	41470.6	4	UniProt (Q9YAZ5) Pfam (PF03463) Pfam (PF03465)	Aeropyrum pernix K1
3	I, K, M, O (A, C, E, G)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	4	Chemie (GTP)
4	J, L, N, P (A, C, E, G)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
5	Q, R, S, T, U... (A, B, C, D, E...)	water	water		18.0	1349	Chemie (HOH)

Sequence modifications

A, C, E, G: 1 - 437 (UniProt: Q9YAV0)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Leu 438	-	expression tag
Ala 439	-	expression tag
Arg 440	-	expression tag
Gly 441	-	expression tag
Ser 442	-	expression tag
Gly 443	-	expression tag
Cys 444	-	expression tag

B, D, F, H: 1 - 356 (UniProt: Q9YAZ5)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	364532.6
Non-Polymers*	Number of molecules	8
	Total formula weight	2189.9
All*	Total formula weight	366722.6

*Water molecules are not included.