Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3WXM

Crystal structure of archaeal Pelota and GTP-bound EF1 alpha complex

Replaces: 3AGJ

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0003746	molecular_function	translation elongation factor activity
A	0003924	molecular_function	GTPase activity
A	0005525	molecular_function	GTP binding
A	0005737	cellular_component	cytoplasm
A	0006412	biological_process	translation
A	0006414	biological_process	translational elongation
A	0016787	molecular_function	hydrolase activity
A	0046872	molecular_function	metal ion binding
B	0004518	molecular_function	nuclease activity
B	0004519	molecular_function	endonuclease activity
B	0005737	cellular_component	cytoplasm
B	0016787	molecular_function	hydrolase activity
B	0032790	biological_process	ribosome disassembly
B	0046872	molecular_function	metal ion binding
B	0070481	biological_process	nuclear-transcribed mRNA catabolic process, non-stop decay
B	0070651	biological_process	nonfunctional rRNA decay
B	0070966	biological_process	nuclear-transcribed mRNA catabolic process, no-go decay
B	0071025	biological_process	RNA surveillance
C	0000166	molecular_function	nucleotide binding
C	0000287	molecular_function	magnesium ion binding
C	0003746	molecular_function	translation elongation factor activity
C	0003924	molecular_function	GTPase activity
C	0005525	molecular_function	GTP binding
C	0005737	cellular_component	cytoplasm
C	0006412	biological_process	translation
C	0006414	biological_process	translational elongation
C	0016787	molecular_function	hydrolase activity
C	0046872	molecular_function	metal ion binding
D	0004518	molecular_function	nuclease activity
D	0004519	molecular_function	endonuclease activity
D	0005737	cellular_component	cytoplasm
D	0016787	molecular_function	hydrolase activity
D	0032790	biological_process	ribosome disassembly
D	0046872	molecular_function	metal ion binding
D	0070481	biological_process	nuclear-transcribed mRNA catabolic process, non-stop decay
D	0070651	biological_process	nonfunctional rRNA decay
D	0070966	biological_process	nuclear-transcribed mRNA catabolic process, no-go decay
D	0071025	biological_process	RNA surveillance
E	0000166	molecular_function	nucleotide binding
E	0000287	molecular_function	magnesium ion binding
E	0003746	molecular_function	translation elongation factor activity
E	0003924	molecular_function	GTPase activity
E	0005525	molecular_function	GTP binding
E	0005737	cellular_component	cytoplasm
E	0006412	biological_process	translation
E	0006414	biological_process	translational elongation
E	0016787	molecular_function	hydrolase activity
E	0046872	molecular_function	metal ion binding
F	0004518	molecular_function	nuclease activity
F	0004519	molecular_function	endonuclease activity
F	0005737	cellular_component	cytoplasm
F	0016787	molecular_function	hydrolase activity
F	0032790	biological_process	ribosome disassembly
F	0046872	molecular_function	metal ion binding
F	0070481	biological_process	nuclear-transcribed mRNA catabolic process, non-stop decay
F	0070651	biological_process	nonfunctional rRNA decay
F	0070966	biological_process	nuclear-transcribed mRNA catabolic process, no-go decay
F	0071025	biological_process	RNA surveillance
G	0000166	molecular_function	nucleotide binding
G	0000287	molecular_function	magnesium ion binding
G	0003746	molecular_function	translation elongation factor activity
G	0003924	molecular_function	GTPase activity
G	0005525	molecular_function	GTP binding
G	0005737	cellular_component	cytoplasm
G	0006412	biological_process	translation
G	0006414	biological_process	translational elongation
G	0016787	molecular_function	hydrolase activity
G	0046872	molecular_function	metal ion binding
H	0004518	molecular_function	nuclease activity
H	0004519	molecular_function	endonuclease activity
H	0005737	cellular_component	cytoplasm
H	0016787	molecular_function	hydrolase activity
H	0032790	biological_process	ribosome disassembly
H	0046872	molecular_function	metal ion binding
H	0070481	biological_process	nuclear-transcribed mRNA catabolic process, non-stop decay
H	0070651	biological_process	nonfunctional rRNA decay
H	0070966	biological_process	nuclear-transcribed mRNA catabolic process, no-go decay
H	0071025	biological_process	RNA surveillance

Functional Information from PDB Data

site_id	AC1
Number of Residues	24
Details	BINDING SITE FOR RESIDUE GTP A 501

Chain	Residue
A	VAL15
A	ALA56
A	ILE70
A	THR71
A	GLY93
A	ASN152
A	LYS153
A	ASP155
A	SER193
A	ALA194
A	TRP195
A	ASP16
A	MG502
A	HOH604
A	HOH606
A	HOH788
A	HOH822
A	HIS17
A	GLY18
A	LYS19
A	SER20
A	THR21
A	SER52
A	PHE53

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 502

Chain	Residue
A	SER20
A	THR71
A	GTP501
A	HOH606
A	HOH822

site_id	AC3
Number of Residues	23
Details	BINDING SITE FOR RESIDUE GTP C 501

Chain	Residue
C	VAL15
C	ASP16
C	HIS17
C	GLY18
C	LYS19
C	SER20
C	THR21
C	SER52
C	ALA56
C	ILE70
C	THR71
C	GLY93
C	ASN152
C	LYS153
C	ASP155
C	SER193
C	ALA194
C	TRP195
C	MG502
C	HOH605
C	HOH616
C	HOH712
C	HOH792

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG C 502

Chain	Residue
C	SER20
C	THR71
C	GTP501
C	HOH712
C	HOH792

site_id	AC5
Number of Residues	24
Details	BINDING SITE FOR RESIDUE GTP E 501

Chain	Residue
E	VAL15
E	ASP16
E	HIS17
E	GLY18
E	LYS19
E	SER20
E	THR21
E	SER52
E	PHE53
E	ALA56
E	ILE70
E	THR71
E	GLY93
E	ASN152
E	LYS153
E	ASP155
E	SER193
E	ALA194
E	TRP195
E	MG502
E	HOH601
E	HOH603
E	HOH606
E	HOH778

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG E 502

Chain	Residue
E	SER20
E	THR71
E	GTP501
E	HOH603
E	HOH778

site_id	AC7
Number of Residues	24
Details	BINDING SITE FOR RESIDUE GTP G 501

Chain	Residue
G	ASN152
G	LYS153
G	ASP155
G	ALA156
G	SER193
G	ALA194
G	TRP195
G	MG502
G	HOH601
G	HOH602
G	HOH724
G	VAL15
G	ASP16
G	HIS17
G	GLY18
G	LYS19
G	SER20
G	THR21
G	SER52
G	PHE53
G	ALA56
G	ILE70
G	THR71
G	GLY93

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG G 502

Chain	Residue
G	SER20
G	THR71
G	GTP501
G	HOH601
G	HOH724

Functional Information from PROSITE/UniProt

site_id	PS00301
Number of Residues	16
Details	G_TR_1 Translational (tr)-type guanine nucleotide-binding (G) domain signature. DKmkeEReRGITIdlT

Chain	Residue	Details
A	ASP60-THR75

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	900
Details	Domain: {"description":"tr-type G"}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	28
Details	Region: {"description":"G1","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	16
Details	Region: {"description":"G2","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	12
Details	Region: {"description":"G3","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	12
Details	Region: {"description":"G4","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	8
Details	Region: {"description":"G5","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	60
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00118","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)