Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-22 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 148.926, 158.944, 427.236 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.433 - 2.300 |
R-factor | 0.2015 |
Rwork | 0.198 |
R-free | 0.26090 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.275 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.300 |
Number of reflections | 216696 |
Completeness [%] | 95.8 |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 2.2M NaCl, 100mM imidazole, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |