3WXG

Crystal structure of CYLD USP domain (C596A) in complex with Lys63-linked diubiquitin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	Uncharacterized protein	polymer	312	35975.5	2	UniProt (E7FEV5) Pfam (PF00443) In PDB	Danio rerio (zebra fish)	CYLD protein
2	B, E	Ubiquitin	polymer	76	8604.8	2	UniProt (P0CG50) Pfam (PF00240) In PDB	Mus musculus (mouse)	distal ubiquitin
3	C, F	Ubiquitin	polymer	72	8192.4	2	UniProt (P0CG50) Pfam (PF00240) In PDB	Mus musculus (mouse)	proximal ubiquitin

Sequence modifications

A, D: 578 - 780 (UniProt: E7FEV5)

PDB	External Database	Details
Ser 575	-	expression tag
His 576	-	expression tag
Met 577	-	expression tag
Ala 596	Cys 596	engineered mutation

A, D: 785 - 886 (UniProt: E7FEV5)

PDB	External Database	Details
Leu 781	-	linker
Glu 782	-	linker
Gly 783	-	linker
Gly 784	-	linker

B, E: 1 - 76 (UniProt: P0CG50)

PDB	External Database	Details
Arg 63	Lys 63	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	105545.5
All*	Total formula weight	105545.5

*Water molecules are not included.