3WXG
Crystal structure of CYLD USP domain (C596A) in complex with Lys63-linked diubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-28 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.017, 65.657, 69.821 |
| Unit cell angles | 103.49, 89.86, 90.61 |
Refinement procedure
| Resolution | 41.890 - 3.100 |
| R-factor | 0.19098 |
| Rwork | 0.188 |
| R-free | 0.24559 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2VHF and 1UBQ |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.363 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 15888 | |
| <I/σ(I)> | 2.06 | |
| Completeness [%] | 96.7 | 91.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2M sodium malonate buffer, 18% PEG3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
