3WXG
Crystal structure of CYLD USP domain (C596A) in complex with Lys63-linked diubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-28 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 |
Unit cell lengths | 50.017, 65.657, 69.821 |
Unit cell angles | 103.49, 89.86, 90.61 |
Refinement procedure
Resolution | 41.890 - 3.100 |
R-factor | 0.19098 |
Rwork | 0.188 |
R-free | 0.24559 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2VHF and 1UBQ |
RMSD bond length | 0.010 |
RMSD bond angle | 1.363 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.150 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 15888 | |
<I/σ(I)> | 2.06 | |
Completeness [%] | 96.7 | 91.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2M sodium malonate buffer, 18% PEG3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |