3WJ8

Crystal Structure of DL-2-haloacid dehalogenase mutant with 2-bromo-2-methylpropionate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, D, E, F...)	DL-2-haloacid dehalogenase	polymer	301	34092.9	6	UniProt (A6BM74)	Methylobacterium
2	G, I, K, M, O... (A, B, D, E, F...)	2-bromo-2-methylpropanoic acid	non-polymer		167.0	6	Chemie (B2P)
3	H, J, L, N, P... (A, B, D, E, F...)	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
4	S, T, U, V, W... (A, B, D, E, F...)	water	water		18.0	611	Chemie (HOH)

Sequence modifications

A, B, D, E, F, G: 1 - 301 (UniProt: A6BM74)

PDB	External Database	Details
His 54	Asp 54	engineered mutation
Asn 194	Asp 194	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	204557.5
Non-Polymers*	Number of molecules	12
	Total formula weight	1554.6
All*	Total formula weight	206112.1

*Water molecules are not included.