3W9C

Crystal structure of the electron transfer complex of cytochrome p450cam with putidaredoxin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Camphor 5-monooxygenase	polymer	416	46665.0	1	UniProt (P00183) Pfam (PF00067) In PDB	Pseudomonas putida	Cytochrome P450-cam, Cytochrome P450cam
2	B	Putidaredoxin	polymer	108	11601.1	1	UniProt (P00259) Pfam (PF00111) In PDB	Pseudomonas putida	PDX
3	A	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	1	Chemie (HEM)
4	A, B	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
5	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6	B	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	1	Chemie (FES)
7		water	water		18.0	35	Chemie (HOH)

Sequence modifications

A: 1 - 414 (UniProt: P00183)

PDB	External Database	Details
Met -1	-	expression tag
Gly 0	-	expression tag
Cys 126	Lys 127	engineered mutation
Cys 130	Arg 131	engineered mutation
Ala 334	Cys 335	engineered mutation

B: 1 - 106 (UniProt: P00259)

PDB	External Database	Details
Met -1	-	expression tag
Gly 0	-	expression tag
Ser 73	Cys 74	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	58266.1
Non-Polymers*	Number of molecules	7
	Total formula weight	1268.7
All*	Total formula weight	59534.7

*Water molecules are not included.