3W9C
Crystal structure of the electron transfer complex of cytochrome p450cam with putidaredoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-03 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.717, 77.991, 60.016 |
| Unit cell angles | 90.00, 95.57, 90.00 |
Refinement procedure
| Resolution | 61.780 - 2.500 |
| R-factor | 0.18829 |
| Rwork | 0.185 |
| R-free | 0.25088 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zwu 1oqr |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.753 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.780 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.098 | 0.440 |
| Number of reflections | 16251 | |
| <I/σ(I)> | 19.4 | 3.6 |
| Completeness [%] | 99.6 | 98.9 |
| Redundancy | 3.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1M HEPES (PH7.5), 0.1M SODIUM CHLORIDE, 1.6M AMMONIUM SULFATE, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
