3W9C

Crystal structure of the electron transfer complex of cytochrome p450cam with putidaredoxin

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004497	molecular_function	monooxygenase activity
A	0005506	molecular_function	iron ion binding
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0016491	molecular_function	oxidoreductase activity
A	0016705	molecular_function	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
A	0018683	molecular_function	camphor 5-monooxygenase activity
A	0019383	biological_process	(+)-camphor catabolic process
A	0020037	molecular_function	heme binding
A	0046872	molecular_function	metal ion binding
B	0005515	molecular_function	protein binding
B	0005829	cellular_component	cytosol
B	0009055	molecular_function	electron transfer activity
B	0022900	biological_process	electron transport chain
B	0046872	molecular_function	metal ion binding
B	0051536	molecular_function	iron-sulfur cluster binding
B	0051537	molecular_function	2 iron, 2 sulfur cluster binding
B	0140647	biological_process	P450-containing electron transport chain

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	BINDING SITE FOR RESIDUE HEM A 501

Chain	Residue
A	GLN108
A	ARG299
A	GLN322
A	THR349
A	PHE350
A	GLY351
A	HIS355
A	CYS357
A	GLY359
A	GOL505
A	ARG112
A	LEU244
A	LEU245
A	GLY248
A	GLY249
A	THR252
A	PHE256
A	ASP297

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 502

Chain	Residue
A	SER267
A	PRO268
A	GLU269
A	HIS270

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 503

Chain	Residue
A	ALA334
A	PRO335
A	MET336
A	HIS337

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 504

Chain	Residue
A	PRO19
A	GLU20
A	HIS21
A	ARG271

---

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL A 505

Chain	Residue
A	GLY248
A	THR252
A	HEM501

---

site_id	AC6
Number of Residues	9
Details	BINDING SITE FOR RESIDUE FES B 201

Chain	Residue
B	GLY37
B	CYS39
B	GLY41
B	ALA43
B	SER44
B	CYS45
B	ALA46
B	CYS48
B	CYS86

---

site_id	AC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 B 202

Chain	Residue
B	HIS8
B	ALA76
B	GLU77
B	ASP103
B	ARG104

---

Functional Information from PROSITE/UniProt

site_id	PS00086
Number of Residues	10
Details	CYTOCHROME_P450 Cytochrome P450 cysteine heme-iron ligand signature. FGhGSHLCLG

Chain	Residue	Details
A	PHE350-GLY359

---

site_id	PS00814
Number of Residues	11
Details	ADX Adrenodoxin family, iron-sulfur binding region signature. CggSaSCATCH

Chain	Residue	Details
B	CYS39-HIS49

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Binding site: {"description":"axial binding residue"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	104
Details	Domain: {"description":"2Fe-2S ferredoxin-type","evidences":[{"source":"PROSITE-ProRule","id":"PRU00465","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00465","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"8204575","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 133

Chain	Residue	Details
A	ARG186	hydrogen bond donor, proton acceptor, proton donor, proton relay
A	ASP251	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay
A	THR252	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay
A	CYS357	electrostatic stabiliser, hydrogen bond acceptor, metal ligand
A	LEU358	electrostatic stabiliser, hydrogen bond donor
A	GLY359	electrostatic stabiliser, hydrogen bond donor

---