3VHL

Crystal structure of the DHR-2 domain of DOCK8 in complex with Cdc42 (T17N mutant)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Dedicator of cytokinesis protein 8	polymer	288	33787.8	1	UniProt (Q8C147) Pfam (PF20422) Pfam (PF20421)	Mus musculus (mouse)
2	B (B)	Cell division control protein 42 homolog	polymer	195	21474.5	1	UniProt (P60953) Pfam (PF00071)	Homo sapiens (human)	Cdc42, G25K GTP-binding protein
3	C, D (A, B)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
4	E, F (A, B)	water	water		18.0	194	Chemie (HOH)

Sequence modifications

A: 1787 - 2067 (UniProt: Q8C147)

PDB	External Database	Details
Gly 1780	-	expression tag
Ser 1781	-	expression tag
Ser 1782	-	expression tag
Gly 1783	-	expression tag
Ser 1784	-	expression tag
Ser 1785	-	expression tag
Gly 1786	-	expression tag

B: 1 - 188 (UniProt: P60953)

PDB	External Database	Details
Gly -6	-	expression tag
Ser -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Asn 17	Thr 17	engineered mutation
Ser 188	Cys 188	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	55262.3
Non-Polymers*	Number of molecules	2
	Total formula weight	189.9
All*	Total formula weight	55452.3

*Water molecules are not included.