3VHL
Crystal structure of the DHR-2 domain of DOCK8 in complex with Cdc42 (T17N mutant)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-15 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 104.185, 73.279, 65.031 |
Unit cell angles | 90.00, 104.82, 90.00 |
Refinement procedure
Resolution | 29.679 - 2.085 |
R-factor | 0.1823 |
Rwork | 0.178 |
R-free | 0.23240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wm9 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.275 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.160 |
High resolution limit [Å] | 2.085 | 2.090 |
Number of reflections | 28080 | |
<I/σ(I)> | 24.6 | 4.96 |
Completeness [%] | 99.5 | 98.4 |
Redundancy | 5.5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.2M Potassium phosphate, 20% PEG 3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |