3UVI
tRNA-guanine transglycosylase C158S C281S W326E E339Q mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Queuine tRNA-ribosyltransferase | polymer | 388 | 42979.6 | 1 | UniProt (P28720) Pfam (PF01702) | Zymomonas mobilis | Guanine insertion enzyme, tRNA-guanine transglycosylase |
| 2 | B (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 3 | C, D, E, F, G... (A) | IODIDE ION | non-polymer | 126.9 | 8 | Chemie (IOD) | |||
| 4 | K, L (A) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 5 | M, N, O, P (A) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 6 | Q (A) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 1 | Chemie (DMS) | |||
| 7 | R (A) | water | water | 18.0 | 479 | Chemie (HOH) |
Sequence modifications
A: 1 - 386 (UniProt: P28720)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ser 158 | Cys 158 | engineered mutation |
| Ser 281 | Cys 281 | engineered mutation |
| Lys 312 | Thr 312 | SEE REMARK 999 |
| Glu 326 | Trp 326 | engineered mutation |
| Gln 339 | Glu 339 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 42979.6 | |
| Non-Polymers* | Number of molecules | 16 |
| Total formula weight | 1598.1 | |
| All* | Total formula weight | 44577.7 |
