3UVI

tRNA-guanine transglycosylase C158S C281S W326E E339Q mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-16
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.91841
Spacegroup name	C 1 2 1
Unit cell lengths	91.019, 64.984, 70.488
Unit cell angles	90.00, 95.60, 90.00

Refinement procedure

Resolution	35.076 - 1.550
R-factor	0.166
Rwork	0.165
R-free	0.19070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pud
RMSD bond length	0.006
RMSD bond angle	1.054
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.580
High resolution limit [Å]	1.550	1.550
Number of reflections	58805
<I/σ(I)>	16.08	2.78
Completeness [%]	99.0	89.7
Redundancy	3	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	291	Sodium iodide 0.2M, PEG 3350 20% w/v, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 291K