3UVI
tRNA-guanine transglycosylase C158S C281S W326E E339Q mutant
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0002099 | biological_process | tRNA wobble guanine modification |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006400 | biological_process | tRNA modification |
| A | 0008033 | biological_process | tRNA processing |
| A | 0008479 | molecular_function | tRNA-guanosine(34) queuine transglycosylase activity |
| A | 0008616 | biological_process | tRNA queuosine(34) biosynthetic process |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016757 | molecular_function | glycosyltransferase activity |
| A | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN A 387 |
| Chain | Residue |
| A | CYS318 |
| A | CYS320 |
| A | CYS323 |
| A | HIS349 |
| site_id | AC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE IOD A 388 |
| Chain | Residue |
| A | LYS264 |
| A | TYR381 |
| site_id | AC3 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE IOD A 389 |
| Chain | Residue |
| A | ALA53 |
| site_id | AC4 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE IOD A 392 |
| Chain | Residue |
| A | ASN301 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE IOD A 393 |
| Chain | Residue |
| A | HIS333 |
| A | GLN339 |
| A | MET93 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE IOD A 394 |
| Chain | Residue |
| A | VAL45 |
| A | SER281 |
| A | PRO284 |
| A | THR285 |
| site_id | AC7 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE CL A 396 |
| Chain | Residue |
| A | HIS333 |
| site_id | AC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CL A 397 |
| Chain | Residue |
| A | ALA163 |
| A | THR164 |
| A | ARG167 |
| A | HOH618 |
| A | HOH657 |
| site_id | AC9 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL A 398 |
| Chain | Residue |
| A | PRO56 |
| A | GLY94 |
| A | TRP95 |
| A | ASP96 |
| A | ARG97 |
| A | HOH664 |
| A | HOH712 |
| site_id | BC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL A 399 |
| Chain | Residue |
| A | ALA61 |
| A | CYS320 |
| A | ALA321 |
| A | GLU348 |
| A | HOH676 |
| A | HOH739 |
| site_id | BC2 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE GOL A 400 |
| Chain | Residue |
| A | PHE14 |
| A | SER15 |
| A | PHE16 |
| A | GLU119 |
| A | ARG174 |
| A | ARG177 |
| A | LYS255 |
| A | HOH459 |
| A | HOH524 |
| A | HOH558 |
| A | HOH746 |
| site_id | BC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL A 401 |
| Chain | Residue |
| A | GLU273 |
| A | ARG274 |
| A | HOH530 |
| A | HOH559 |
| site_id | BC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE DMS A 402 |
| Chain | Residue |
| A | MET172 |
| A | MET176 |
| A | GLN213 |
| A | ALA217 |
| A | HOH637 |
| A | HOH709 |
| A | HOH801 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 6 |
| Details | Region: {"description":"RNA binding","evidences":[{"source":"HAMAP-Rule","id":"MF_00168","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12949492","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | Region: {"description":"RNA binding; important for wobble base 34 recognition","evidences":[{"source":"HAMAP-Rule","id":"MF_00168","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12949492","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_00168","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12949492","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Nucleophile","evidences":[{"source":"HAMAP-Rule","id":"MF_00168","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12949492","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 7 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00168","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12949492","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00168","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"10413112","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11178905","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11921407","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12646024","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12909636","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12949492","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"14523925","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19627989","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8654383","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8961936","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 6 |
| Details | M-CSA 881 |
| Chain | Residue | Details |
| A | ASP102 | proton shuttle (general acid/base) |
| A | ASP280 | covalent catalysis |
| A | CYS318 | metal ligand |
| A | CYS320 | metal ligand |
| A | CYS323 | metal ligand |
| A | HIS349 | metal ligand |
