3UEZ

Crystal structure of the human Colony-Stimulating Factor 1 (hCSF-1) cytokine in complex with the viral receptor BARF1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Secreted protein BARF1	polymer	208	23412.7	4	UniProt (P03228) Pfam (PF22305)	Human herpesvirus 4 (HHV-4)	33 kDa early protein, p33
2	E, F, G, H (E, F, G, H)	Macrophage colony-stimulating factor 1	polymer	153	17868.2	4	UniProt (P09603) Pfam (PF05337)	Homo sapiens (human)	CSF-1, M-CSF, MCSF, Lanimostim, Processed macrophage colony-stimulating factor 1
3	I, J, K, L (I, J, K, L)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	4	In PDB GlyTouCan (G15407YE)

Sequence modifications

A, B, C, D: 21 - 221 (UniProt: P03228)

PDB	External Database	Details
Ser 169	Thr 169	engineered mutation
Lys 222	-	expression tag
His 223	-	expression tag
His 224	-	expression tag
His 225	-	expression tag
His 226	-	expression tag
His 227	-	expression tag
His 228	-	expression tag

E, F, G, H: 1 - 149 (UniProt: P09603)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	165123.5
Branched	Number of molecules	4
	Total formula weight	2346.2
All*	Total formula weight	167469.7

*Water molecules are not included.