3UEZ
Crystal structure of the human Colony-Stimulating Factor 1 (hCSF-1) cytokine in complex with the viral receptor BARF1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9714 |
| Spacegroup name | H 3 |
| Unit cell lengths | 235.240, 235.240, 95.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 3.414 |
| R-factor | 0.2336 |
| Rwork | 0.231 |
| R-free | 0.27350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.643 |
| Refinement software | PHENIX ((phenix.refine: dev_874)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.810 | 3.500 |
| High resolution limit [Å] | 3.400 | 3.410 |
| Number of reflections | 25862 | |
| Completeness [%] | 96.4 | 90.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris HCl, 1.0M ammonium sulphate, pH 8.50, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
