3UBT
Crystal Structure of C71S Mutant of DNA Cytosine-5 Methyltransferase M.HaeIII Bound to DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | Y, A, B | Modification methylase HaeIII | polymer | 331 | 37837.3 | 3 | UniProt (P20589) Pfam (PF00145) In PDB | Haemophilus aegyptius (Koch-Weeks bacillus) | M.HaeIII, Cytosine-specific methyltransferase HaeIII |
| 2 | D, E, F, G, H... | 5'-D(*TP*GP*GP*CP*CP*A)-3' | polymer | 6 | 1809.2 | 6 | |||
| 3 | Y, A, B | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 4 | Y, A | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 3 | Chemie (ATP) | |||
| 5 | Y, A, B | NONAETHYLENE GLYCOL | non-polymer | 414.5 | 3 | Chemie (2PE) | |||
| 6 | water | water | 18.0 | 568 | Chemie (HOH) |
Sequence modifications
Y, A, B: 1 - 330 (UniProt: P20589)
| PDB | External Database | Details |
|---|---|---|
| Ser 71 | Cys 71 | engineered mutation |
| Asn 331 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 124367.3 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 2871.4 | |
| All* | Total formula weight | 127238.7 |
