3UBT
Crystal Structure of C71S Mutant of DNA Cytosine-5 Methyltransferase M.HaeIII Bound to DNA
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0003677 | molecular_function | DNA binding |
| A | 0003886 | molecular_function | DNA (cytosine-5-)-methyltransferase activity |
| A | 0005524 | molecular_function | ATP binding |
| A | 0008168 | molecular_function | methyltransferase activity |
| A | 0009307 | biological_process | DNA restriction-modification system |
| A | 0016740 | molecular_function | transferase activity |
| A | 0032259 | biological_process | methylation |
| A | 0044027 | biological_process | negative regulation of gene expression via chromosomal CpG island methylation |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0003677 | molecular_function | DNA binding |
| B | 0003886 | molecular_function | DNA (cytosine-5-)-methyltransferase activity |
| B | 0005524 | molecular_function | ATP binding |
| B | 0008168 | molecular_function | methyltransferase activity |
| B | 0009307 | biological_process | DNA restriction-modification system |
| B | 0016740 | molecular_function | transferase activity |
| B | 0032259 | biological_process | methylation |
| B | 0044027 | biological_process | negative regulation of gene expression via chromosomal CpG island methylation |
| Y | 0000166 | molecular_function | nucleotide binding |
| Y | 0003677 | molecular_function | DNA binding |
| Y | 0003886 | molecular_function | DNA (cytosine-5-)-methyltransferase activity |
| Y | 0005524 | molecular_function | ATP binding |
| Y | 0008168 | molecular_function | methyltransferase activity |
| Y | 0009307 | biological_process | DNA restriction-modification system |
| Y | 0016740 | molecular_function | transferase activity |
| Y | 0032259 | biological_process | methylation |
| Y | 0044027 | biological_process | negative regulation of gene expression via chromosomal CpG island methylation |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CL Y 332 |
| Chain | Residue |
| H | DC11 |
| Y | SER241 |
| site_id | AC2 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE ATP Y 333 |
| Chain | Residue |
| Y | HOH359 |
| Y | HOH368 |
| Y | HOH461 |
| Y | HOH491 |
| Y | HOH501 |
| A | SER258 |
| A | LYS259 |
| Y | GLU29 |
| Y | TYR30 |
| Y | ASP50 |
| Y | ILE51 |
| Y | LEU90 |
| site_id | AC3 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE ATP Y 334 |
| Chain | Residue |
| B | GLU29 |
| B | TYR30 |
| B | ASP50 |
| B | ILE51 |
| B | PRO70 |
| B | LEU90 |
| B | LYS302 |
| Y | SER258 |
| Y | LYS259 |
| Y | ASN260 |
| Y | HOH344 |
| Y | HOH424 |
| Y | HOH463 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE 2PE Y 335 |
| Chain | Residue |
| B | PRO192 |
| B | GLN250 |
| Y | LYS182 |
| Y | TRP186 |
| Y | TRP231 |
| Y | ASN232 |
| site_id | AC5 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CL A 332 |
| Chain | Residue |
| A | SER241 |
| F | DC11 |
| site_id | AC6 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE ATP A 333 |
| Chain | Residue |
| A | GLU29 |
| A | TYR30 |
| A | ASP50 |
| A | ILE51 |
| A | ASN306 |
| A | HOH361 |
| A | HOH457 |
| A | HOH524 |
| A | HOH565 |
| B | LYS259 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE 2PE A 334 |
| Chain | Residue |
| A | LYS182 |
| A | TRP186 |
| Y | PRO192 |
| Y | PRO194 |
| site_id | AC8 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE CL B 332 |
| Chain | Residue |
| B | SER241 |
| C | DC11 |
| Y | HOH479 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE 2PE B 333 |
| Chain | Residue |
| A | PRO194 |
| A | ALA195 |
| B | LYS182 |
| B | TRP186 |
| B | TRP231 |
Functional Information from PROSITE/UniProt
| site_id | PS00095 |
| Number of Residues | 19 |
| Details | C5_MTASE_2 C-5 cytosine-specific DNA methylases C-terminal signature. KmiGNAVpVnLayeIaktI |
| Chain | Residue | Details |
| Y | LYS302-ILE320 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 978 |
| Details | Domain: {"description":"SAM-dependent MTase C5-type","evidences":[{"source":"PROSITE-ProRule","id":"PRU01016","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 3 |
| Details | Active site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01016","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10018","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"1932026","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 9 |
| Details | Binding site: {"evidences":[{"source":"PDB","id":"3UBT","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
