3U60

Structure of T4 Bacteriophage Clamp Loader Bound To Open Clamp, DNA and ATP Analog

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (B, C, D, E)	DNA polymerase accessory protein 44	polymer	324	36189.6	4	UniProt (P04526) Pfam (PF00004) Pfam (PF21328) Pfam (PF21429)	Enterobacteria phage T4	Clamp loader large subunit, Protein Gp44
2	E (I)	Template DNA strand	polymer	30	9172.9	1
3	F (J)	Primer DNA strand	polymer	20	6136.0	1
4	G (A)	DNA polymerase accessory protein 62	polymer	195	22388.7	1	UniProt (P04527) Pfam (PF16790)	Enterobacteria phage T4	Clamp loader small subunit, Protein Gp62
5	H, I, J (G, H, F)	DNA polymerase processivity component	polymer	228	25162.6	3	UniProt (P04525) Pfam (PF02916) Pfam (PF09116)	Enterobacteria phage T4	DNA polymerase accessory protein 45, Gp45
6	K, M, O (B, C, D)	[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium	non-polymer		492.2	3	Chemie (08T)
7	L, N, P, R (B, C, D, E)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
8	Q (E)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)

Sequence modifications

B, C, D, E: 1 - 319 (UniProt: P04526)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

A: 2 - 187 (UniProt: P04527)

PDB	External Database	Details
Gly 188	-	expression tag
Leu 189	-	expression tag
Glu 190	-	expression tag
His 191	-	expression tag
His 192	-	expression tag
His 193	-	expression tag
His 194	-	expression tag
His 195	-	expression tag
His 196	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	257943.8
Non-Polymers*	Number of molecules	8
	Total formula weight	2001.0
All*	Total formula weight	259944.8

*Water molecules are not included.