3U60
Structure of T4 Bacteriophage Clamp Loader Bound To Open Clamp, DNA and ATP Analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.518, 118.443, 133.078 |
| Unit cell angles | 90.00, 102.06, 90.00 |
Refinement procedure
| Resolution | 49.709 - 3.340 |
| R-factor | 0.246 |
| Rwork | 0.244 |
| R-free | 0.28170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.869 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.490 |
| High resolution limit [Å] | 3.340 | 3.340 |
| Number of reflections | 40789 | |
| <I/σ(I)> | 9 | 2.2 |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 4.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 295 | 20% PEG4k, 0.1M Tris pH7.5, 10mM MgCl2, 20mM NaCl, VAPOR DIFFUSION, temperature 295K |
