3U5Z

Structure of T4 Bacteriophage clamp loader bound to the T4 clamp, primer-template DNA, and ATP analog

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, C, D, E, L...	DNA polymerase accessory protein 44	polymer	324	36189.6	8	UniProt (P04526) Pfam (PF00004) Pfam (PF21328) Pfam (PF21429) In PDB	Enterobacteria phage T4	Clamp loader large subunit, Protein Gp44
2	A, K	DNA polymerase accessory protein 62	polymer	199	22941.3	2	UniProt (P04527) Pfam (PF16790) In PDB	Enterobacteria phage T4	Clamp loader small subunit, Protein Gp62
3	G, H, F, Q, R...	DNA polymerase processivity component	polymer	228	25162.6	6	UniProt (P04525) Pfam (PF02916) Pfam (PF09116) In PDB	Enterobacteria phage T4	DNA polymerase accessory protein 45, Gp45
4	I, S	Template DNA strand	polymer	30	9172.9	2
5	J, T	Primer DNA strand	polymer	20	6136.0	2
6	L, M, N, B, C...	[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium	non-polymer		492.2	6	Chemie (08T)
7	E, L, M, N, O...	MAGNESIUM ION	non-polymer		24.3	8	Chemie (MG)
8	E, O	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)

Sequence modifications

B, C, D, E, L, M, N, O: 1 - 319 (UniProt: P04526)

PDB	External Database	Details
Gly -4	-	EXPRESSION TAG
Pro -3	-	EXPRESSION TAG
Gly -2	-	EXPRESSION TAG
Gly -1	-	EXPRESSION TAG
Ser 0	-	EXPRESSION TAG

A, K: 2 - 187 (UniProt: P04527)

PDB	External Database	Details
Gly 188	-	EXPRESSION TAG
Leu 189	-	EXPRESSION TAG
Glu 190	-	EXPRESSION TAG
His 191	-	EXPRESSION TAG
His 192	-	EXPRESSION TAG
His 193	-	EXPRESSION TAG
His 194	-	EXPRESSION TAG
His 195	-	EXPRESSION TAG
His 196	-	EXPRESSION TAG
His 197	-	EXPRESSION TAG
His 198	-	EXPRESSION TAG
His 199	-	EXPRESSION TAG
His 200	-	EXPRESSION TAG

Contents of the asymmetric unit

Polymers	Number of chains	20
	Total formula weight	516992.8
Non-Polymers*	Number of molecules	16
	Total formula weight	4002.0
All*	Total formula weight	520994.8

*Water molecules are not included.