3U5Z
Structure of T4 Bacteriophage clamp loader bound to the T4 clamp, primer-template DNA, and ATP analog
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 95.702, 239.177, 247.672 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.193 - 3.500 |
R-factor | 0.2322 |
Rwork | 0.231 |
R-free | 0.27870 |
Structure solution method | MRSAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.678 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.560 |
High resolution limit [Å] | 3.500 | 3.500 |
Number of reflections | 137285 | |
<I/σ(I)> | 9.3 | 2.1 |
Completeness [%] | 99.2 | 90.7 |
Redundancy | 5.1 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 9% PEG4k, 0.1M MES pH 6.5, 50mM NaCl, 25mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 288K |