3U28

Crystal structure of a Cbf5-Nop10-Gar1 complex from Saccharomyces cerevisiae

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	H/ACA ribonucleoprotein complex subunit 4	polymer	400	44981.8	1	UniProt (P33322) Pfam (PF08068) Pfam (PF01509) Pfam (PF16198) Pfam (PF01472)	Saccharomyces cerevisiae (yeast)	Centromere-binding factor 5, Centromere/microtubule-binding protein CBF5, H/ACA snoRNP protein CBF5, Small nucleolar RNP protein CBF5, p64'
2	B (B)	H/ACA ribonucleoprotein complex subunit 3	polymer	58	6649.7	1	UniProt (Q6Q547) Pfam (PF04135)	Saccharomyces cerevisiae (yeast)	Nucleolar protein 10, Nucleolar protein family A member 3, snoRNP protein NOP10
3	C (C)	H/ACA ribonucleoprotein complex subunit 1	polymer	114	12618.4	1	UniProt (P28007) Pfam (PF04410)	Saccharomyces cerevisiae (yeast)	snoRNP protein GAR1
4	D, E, F (A, B, C)	water	water		18.0	279	Chemie (HOH)

Sequence modifications

A: 3 - 394 (UniProt: P33322)

PDB	External Database	Details
Met 1	-	expression tag
Ala 2	-	expression tag
His 395	-	expression tag
His 396	-	expression tag
His 397	-	expression tag
His 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag

C: 32 - 124 (UniProt: P28007)

PDB	External Database	Details
Met 11	-	expression tag
Gly 12	-	expression tag
Ser 13	-	expression tag
Ser 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
Ser 21	-	expression tag
Ser 22	-	expression tag
Gly 23	-	expression tag
Leu 24	-	expression tag
Val 25	-	expression tag
Pro 26	-	expression tag
Arg 27	-	expression tag
Gly 28	-	expression tag
Ser 29	-	expression tag
His 30	-	expression tag
Met 31	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	64250.0
All*	Total formula weight	64250.0

*Water molecules are not included.