3U28
Crystal structure of a Cbf5-Nop10-Gar1 complex from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-17 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.413, 59.386, 169.212 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.920 - 1.900 |
| R-factor | 0.20509 |
| Rwork | 0.204 |
| R-free | 0.23343 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hvy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.314 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.109 | 0.432 |
| Number of reflections | 39859 | |
| <I/σ(I)> | 26.5 | 4.2 |
| Completeness [%] | 89.5 | 78.8 |
| Redundancy | 11.2 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.04 | 293 | 0.2M sodium malonate, 7%(w/v) polyethylene glycol 3350, pH 8.04, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
