3TYK

Crystal structure of aminoglycoside phosphotransferase APH(4)-Ia

Replaces:  3OVC

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Hygromycin-B 4-O-kinase	polymer	362	40940.2	1	UniProt (P00557) Pfam (PF01636) In PDB	Escherichia coli	APH(4), Hygromycin B phosphotransferase, Hygromycin-B kinase
2	A	HYGROMYCIN B VARIANT	non-polymer		527.5	1	Chemie (HY0)
3	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4		water	water		18.0	284	Chemie (HOH)

Sequence modifications

A: 1 - 341 (UniProt: P00557)

PDB	External Database	Details
Mse -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Arg -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	40940.2
Non-Polymers*	Number of molecules	2
	Total formula weight	563.0
All*	Total formula weight	41503.2

*Water molecules are not included.