3SPA

Crystal Structure of Human Mitochondrial RNA Polymerase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA-directed RNA polymerase, mitochondrial	polymer	1134	128736.0	1	UniProt (O00411) Pfam (PF14700) Pfam (PF00940) In PDB	Homo sapiens (human)	MtRPOL
2	B	Nonamer peptide	polymer	9	784.0	1		Homo sapiens (human)
3	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	A	CHLORIDE ION	non-polymer		35.5	5	Chemie (CL)
5	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
6		water	water		18.0	145	Chemie (HOH)

Sequence modifications

A: 105 - 1230 (UniProt: O00411)

PDB	External Database	Details
Met 97	-	expression tag
Gly 98	-	expression tag
His 99	-	expression tag
His 100	-	expression tag
His 101	-	expression tag
His 102	-	expression tag
His 103	-	expression tag
His 104	-	expression tag
Ala 555	Glu 555	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	129520.0
Non-Polymers*	Number of molecules	8
	Total formula weight	457.5
All*	Total formula weight	129977.5

*Water molecules are not included.