3SPA
Crystal Structure of Human Mitochondrial RNA Polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 63 |
| Detector technology | CCD |
| Collection date | 2011-02-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 41 |
| Unit cell lengths | 211.258, 211.258, 60.461 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.128 - 2.500 |
| R-factor | 0.1878 |
| Rwork | 0.185 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qln truncated to polyAlanine residues 246-354 392-568 691-741 766-883 of model used |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.120 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.128 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.047 | 0.631 |
| Number of reflections | 46507 | |
| <I/σ(I)> | 15.9 | 1.9 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 12% PEG4000, 10% Glycerol, 30mM Mes 6.5, 60mM (NH4)2SO4, 40mM Tris 8.0, 20mM DTT in situ proteolysed with ArgC (1:1000 w/w ratio) and micro-seeded, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
