3S8R

Crystal Structures of Glutaryl 7-Aminocephalosporanic Acid Acylase: Insight into Autoproteolytic Activation

Replaces:  1OQZ

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Glutaryl-7-aminocephalosporanic-acid acylase	polymer	697	77581.0	2	UniProt (P07662) Pfam (PF01804) In PDB	Pseudomonas	Glutaryl-7-ACA acylase, 7-beta-(4-carboxybutanamido)cephalosporanic acid acylase, GL-7-ACA acylase, GCA, Glutaryl-7-aminocephalosporanic-acid acylase subunit alpha, Glutaryl-7-ACA acylase subunit alpha, Glutaryl-7-aminocephalosporanic-acid acylase subunit beta, Glutaryl-7-ACA acylase subunit beta
2	A, B	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
3		water	water		18.0	878	Chemie (HOH)

Sequence modifications

A, B: 1 - 691 (UniProt: P07662)

PDB	External Database	Details
Asp 125	Glu 154	SEE REMARK 999
Ala 170	Ser 199	engineered mutation
His 692	-	expression tag
His 693	-	expression tag
His 694	-	expression tag
His 695	-	expression tag
His 696	-	expression tag
His 697	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	155162.0
Non-Polymers*	Number of molecules	2
	Total formula weight	184.2
All*	Total formula weight	155346.2

*Water molecules are not included.