3S8R
Crystal Structures of Glutaryl 7-Aminocephalosporanic Acid Acylase: Insight into Autoproteolytic Activation
Replaces: 1OQZFunctional Information from GO Data
Chain | GOid | namespace | contents |
A | 0016787 | molecular_function | hydrolase activity |
A | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
A | 0017000 | biological_process | antibiotic biosynthetic process |
A | 0033968 | molecular_function | glutaryl-7-aminocephalosporanic-acid acylase activity |
A | 0042597 | cellular_component | periplasmic space |
A | 0046677 | biological_process | response to antibiotic |
B | 0016787 | molecular_function | hydrolase activity |
B | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
B | 0017000 | biological_process | antibiotic biosynthetic process |
B | 0033968 | molecular_function | glutaryl-7-aminocephalosporanic-acid acylase activity |
B | 0042597 | cellular_component | periplasmic space |
B | 0046677 | biological_process | response to antibiotic |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 8 |
Details | BINDING SITE FOR RESIDUE GOL A 698 |
Chain | Residue |
A | THR76 |
A | LEU148 |
A | PRO153 |
A | ASP164 |
A | TYR199 |
A | HOH813 |
A | HOH893 |
A | HOH1021 |
site_id | AC2 |
Number of Residues | 6 |
Details | BINDING SITE FOR RESIDUE GOL B 698 |
Chain | Residue |
B | ARG143 |
B | LEU148 |
B | TYR199 |
B | HOH746 |
B | HOH938 |
B | THR76 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 2 |
Details | ACT_SITE: Nucleophile |
Chain | Residue | Details |
A | ALA170 | |
B | ALA170 |
site_id | SWS_FT_FI2 |
Number of Residues | 4 |
Details | ACT_SITE: ACT_SITE => ECO:0000255 |
Chain | Residue | Details |
A | HIS192 | |
A | GLU624 | |
B | HIS192 | |
B | GLU624 |
Catalytic Information from CSA
site_id | CSA1 |
Number of Residues | 1 |
Details | a catalytic site defined by CSA, PubMed 11080627, 11755403 |
Chain | Residue | Details |
A | SER170 |
site_id | MCSA1 |
Number of Residues | 5 |
Details | M-CSA 288 |
Chain | Residue | Details |
A | ALA170 | activator, covalently attached, hydrogen bond acceptor, hydrogen bond donor, increase electrophilicity, nucleofuge, nucleophile, polar interaction, proton acceptor, proton donor |
A | HIS192 | activator, electrostatic stabiliser, increase acidity, steric role |
A | VAL239 | electrostatic stabiliser |
A | ASN413 | electrostatic stabiliser |
A | GLU624 | activator, electrostatic stabiliser, increase acidity, steric role |
site_id | MCSA2 |
Number of Residues | 5 |
Details | M-CSA 288 |
Chain | Residue | Details |
B | ALA170 | activator, covalently attached, hydrogen bond acceptor, hydrogen bond donor, increase electrophilicity, nucleofuge, nucleophile, polar interaction, proton acceptor, proton donor |
B | HIS192 | activator, electrostatic stabiliser, increase acidity, steric role |
B | VAL239 | electrostatic stabiliser |
B | ASN413 | electrostatic stabiliser |
B | GLU624 | activator, electrostatic stabiliser, increase acidity, steric role |